Impedance of the Grape Berry Cuticle as a Novel Phenotypic Trait to Estimate Resistance to Botrytis Cinerea

Sensors

Katja Herzog¹  Rolf Wind²  Reinhard Töpfer² 

[1] Julius Kühn-Institut-Federal Research Centre of Cultivated Plants, Institute for Grapevine Breeding Geilweilerhof, Siebeldingen 76833, Germany;

关键词: sensor development;    phenotyping;    grapevine breeding;    berry skin;    objective data;    bunch compactness;    Vitis vinifera;   

DOI  :  10.3390/s150612498

来源: mdpi

【 摘 要 】

Warm and moist weather conditions during berry ripening provoke Botrytis cinerea (B. cinerea) causing notable bunch rot on susceptible grapevines with the effect of reduced yield and wine quality. Resistance donors of genetic loci to increase B. cinerea resistance are widely unknown. Promising traits of resistance are represented by physical features like the thickness and permeability of the grape berry cuticle. Sensor-based phenotyping methods or genetic markers are rare for such traits. In the present study, the simple-to-handle I-sensor was developed. The sensor enables the fast and reliable measurement of electrical impedance of the grape berry cuticles and its epicuticular waxes (CW). Statistical experiments revealed highly significant correlations between relative impedance of CW and the resistance of grapevines to B. cinerea. Thus, the relative impedance Z_rel of CW was identified as the most important phenotypic factor with regard to the prediction of grapevine resistance to B. cinerea. An ordinal logistic regression analysis revealed a R²_McFadden of 0.37 and confirmed the application of Z_rel of CW for the prediction of bunch infection and in this way as novel phenotyping trait. Applying the I-sensor, a preliminary QTL region was identified indicating that the novel phenotypic trait is as well a valuable tool for genetic analyses.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.