Single Cell Oil Producing Yeasts Lipomyces starkeyi and Rhodosporidium toruloides: Selection of Extraction Strategies and Biodiesel Property Prediction

Energies

Nemailla Bonturi¹  Leonidas Matsakas²  Robert Nilsson²  Paul Christakopoulos²  Everson Alves Miranda¹  Kris Arvid Berglund²  Ulrika Rova² 

[1] Department of Materials and Bioprocess Engineering, School of Chemical Engineering, State University of Campinas, Campinas 13083852, Brazil; E-Mails:;Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources, Luleå University of Technology, Luleå SE 97187, Sweden; E-Mails:

关键词: single cell oil;    lipid extraction;    biodiesel;    Lipomyces starkeyi;    Rhodosporidium toruloides;   

DOI  :  10.3390/en8065040

来源: mdpi

【 摘 要 】

Single cell oils (SCOs) are considered potential raw material for the production of biodiesel. Rhodosporidium sp. and Lipomyces sp. are good candidates for SCO production. Lipid extractability differs according to yeast species and literature on the most suitable method for each oleaginous yeast species is scarce. This work aimed to investigate the efficiency of the most cited strategies for extracting lipids from intact and pretreated cells of Rhodosporidium toruloides and Lipomyces starkeyi. Lipid extractions were conducted using hexane or combinations of chloroform and methanol. The Folch method resulted in the highest lipid yields for both yeasts (42% for R. toruloides and 48% for L. starkeyi). Also, this method eliminates the cell pretreatment step. The Bligh and Dyer method underestimated the lipid content in the tested strains (25% for R. toruloides and 34% for L. starkeyi). Lipid extractability increased after acid pretreatment for the Pedersen, hexane, and Bligh and Dyer methods. For R. toruloides unexpected fatty acid methyl esters (FAME) composition were found for some lipid extraction strategies tested. Therefore, this work provides useful information for analytical and process development aiming at biodiesel production from the SCO of these two yeast species.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.