Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia

International Journal of Molecular Sciences

Colby A. Souders²  Sharon E. Maynard⁴  Jing Yan¹  Yang Wang²  Naomi K. Boatright²  Jessica Sedan²  David Balyozian²  Peter S. Cheslock²  Deborah C. Molrine²  Tiffany A. Moore Simas³ 

[1] Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA; E-Mail:;MassBiologics of the University of Massachusetts Medical School, Boston, MA 02126, USA; E-Mails:;Department of Obstetrics and Gynecology, University of Massachusetts Medical School/UMass Memorial Health Care, Worcester, MA 01605, USA; E-Mail:;Department of Medicine, Division of Nephrology, Lehigh Valley Health Network, University of South Florida Morsani College of Medicine, Allentown, PA 18105, USA; E-Mail:

关键词: preeclampsia;    soluble fms-like tyrosine kinase 1 (sFlt1);    splice variants;    isoforms;    monoclonal antibody (mAb);    diagnostic;   

DOI  :  10.3390/ijms160612436

来源: mdpi

【 摘 要 】

Angiogenic biomarkers, including soluble fms-like tyrosine kinase 1 (sFlt1), are thought to be predictors of preeclampsia onset; however, improvement is needed before a widespread diagnostic test can be utilized. Here we describe the development and use of diagnostic monoclonal antibodies specific to the two main splice variants of sFlt1, sFlt1-1 and sFlt1-14. These antibodies were selected for their sensitivity and specificity to their respective sFlt1 isoform in a capture ELISA format. Data from this pilot study suggest that sFlt1-1 may be more predictive of preeclampsia than total sFlt1. It may be possible to improve current diagnostic platforms if more specific antibodies are utilized.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.