Synthesis of Phospholipid-Protein Conjugates as New Antigens for Autoimmune Antibodies

Molecules

Arindam Maity²  Claudia Macaubas¹  Elizabeth Mellins¹  Kira Astakhova² 

[1]Divisions of Human Gene Therapy and Pediatric Rheumatology, Program in Immunology, Stanford University School of Medicine, 269 Campus Drive, Stanford, MC 5164, USA

[2] E-Mails:

[3]Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, Odense 5230, Denmark

[4] E-Mail:

关键词: CuAAC click chemistry;    antiphospholipid syndrome;    antigens;    β2-glycoprotein I;    phosphoethanolamine;    prothrombin;   

DOI  :  10.3390/molecules200610253

来源: mdpi

【 摘 要 】

Copper(I)-catalyzed azide-alkyne cycloaddition, or CuAAC click chemistry, is an efficient method for bioconjugation aiming at chemical and biological applications. Herein, we demonstrate how the CuAAC method can provide novel phospholipid-protein conjugates with a high potential for the diagnostics and therapy of autoimmune conditions. In doing this, we, for the first time, covalently bind via 1,2,3-triazole linker biologically complementary molecules, namely phosphoethanol amine with human β2-glycoprotein I and prothrombin. The resulting phospholipid-protein conjugates show high binding affinity and specificity for the autoimmune antibodies against autoimmune complexes. Thus, the development of this work might become a milestone in further diagnostics and therapy of autoimmune diseases that involve the production of autoantibodies against the aforementioned phospholipids and proteins, such as antiphospholipid syndrome and systemic lupus erythematosus.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.