Production of Calcaride A by Calcarisporium sp. in Shaken Flasks and Stirred Bioreactors

Marine Drugs

Anu Tamminen¹  Yanming Wang¹  Marilyn G. Wiebe² 

[1] VTT Technical Research Centre of Finland, P.O. Box 1000, FI-02044 VTT, Finland;

关键词: marine fungi;    Calcarisporium;    calcaride A;    stirred tank bioreactor;    pH;    macrocylic polyester;   

DOI  :  10.3390/md13073992

来源: mdpi

【 摘 要 】

Increased interest in marine resources has led to increased screening of marine fungi for novel bioactive compounds and considerable effort is being invested in discovering these metabolites. For compound discovery, small-scale cultures are adequate, but agitated bioreactors are desirable for larger-scale production. Calcarisporium sp. KF525 has recently been described to produce calcaride A, a cyclic polyester with antibiotic activity, in agitated flasks. Here, we describe improvements in the production of calcaride A in both flasks (13-fold improvement) and stirred bioreactors (200-fold improvement). Production of calcaride A in bioreactors was initially substantially lower than in shaken flasks. The cultivation pH (reduced from 6.8 to <5.4), carbon source (sucrose replacing glucose), C/N ratio and nature of mycelial growth (pellets or filaments) were important in improving calcaride A production. Up to 4.5 mg·g⁻¹ biomass (85 mg·L⁻¹) calcaride A were produced in the bioreactor, which was only slightly less than in shaken flasks (14 mg·g⁻¹, 100 mg·L⁻¹). The results demonstrate that a scalable process for calcaride A production could be developed using an iterative approach with flasks and bioreactors.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.