International Journal of Environmental Research and Public Health | |
Quantification of Protozoa and Viruses from Small Water Volumes | |
J. Alfredo Bonilla1  Tonya D. Bonilla1  Amir M. Abdelzaher1  Troy M. Scott1  Jerzy Lukasik2  Helena M. Solo-Gabriele1  Carol J. Palmer1  | |
[1] Oceans and Human Health Center, University of Miami, Key Biscayne, FL 33149, USA; E-Mails:;BCS Laboratories, Inc., Gainesville FL 32609, USA; E-Mail: | |
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DOI : 10.3390/ijerph120707118 | |
来源: mdpi | |
【 摘 要 】
Large sample volumes are traditionally required for the analysis of waterborne pathogens. The need for large volumes greatly limits the number of samples that can be processed. The goals of this study were to compare extraction and detection procedures for quantifying protozoan parasites and viruses from small volumes of marine water. The intent was to evaluate a logistically simpler method of sample collection and processing that would facilitate direct pathogen measures as part of routine monitoring programs. Samples were collected simultaneously using a bilayer device with protozoa capture by size (top filter) and viruses capture by charge (bottom filter). Protozoan detection technologies utilized for recovery of
【 授权许可】
CC BY
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
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RO202003190011007ZK.pdf | 269KB | download |