Molecularly Imprinted Composite Membranes for Selective Detection of 2-Deoxyadenosine in Urine Samples

International Journal of Molecular Sciences

Sonia Scorrano¹  Lucia Mergola²  Maria Pia Di Bello²  Maria Rosaria Lazzoi²  Giuseppe Vasapollo²  Roberta Del Sole² 

[1] Dipartimento di Ingegneria dell’Innovazione, Università del Salento, via per Arnesano Km 1, Lecce 73100, Italy;

关键词: 2-deoxyadenosine;    molecularly imprinted membrane (MIM);    urine detection;    selective separation;   

DOI  :  10.3390/ijms160613746

来源: mdpi

【 摘 要 】

An important challenge for scientific research is the production of artificial systems able to mimic the recognition mechanisms occurring at the molecular level in living systems. A valid contribution in this direction resulted from the development of molecular imprinting. In this work, a novel molecularly imprinted polymer composite membrane (MIM) was synthesized and employed for the selective detection in urine samples of 2-deoxyadenosine (2-dA), an important tumoral marker. By thermal polymerization, the 2-dA-MIM was cross-linked on the surface of a polyvinylidene-difluoride (PVDF) membrane. By characterization techniques, the linking of the imprinted polymer on the surface of the membrane was found. Batch-wise guest binding experiments confirmed the absorption capacity of the synthesized membrane towards the template molecule. Subsequently, a time-course of 2-dA retention on membrane was performed and the best minimum time (30 min) to bind the molecule was established. HPLC analysis was also performed to carry out a rapid detection of target molecule in urine sample with a recovery capacity of 85%. The experiments indicated that the MIM was highly selective and can be used for revealing the presence of 2-dA in urine samples.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.