Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances

Biosensors

Yoshio Suzuki²  Kenji Yokoyama¹ 

[1] School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan; E-Mail:;Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan

关键词: molecular probes;    sensors;    metal ions;    proteins;    DNA;   

DOI  :  10.3390/bios5020337

来源: mdpi

【 摘 要 】

This review is confined to sensors that use fluorescence to transmit biochemical information. Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors. Parameters that define the application of such sensors include intensity, decay time, anisotropy, quenching efficiency, and luminescence energy transfer. To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades. This review describes the immense variety of fluorescent probes that have been designed for the recognitions of ions, small and large molecules, and their biological applications in terms of intracellular fluorescent imaging techniques.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.