Phytochemical Profiling and Evaluation of Pharmacological Activities of Hypericum scabrum L.

Molecules

Lan Jiang²  Sodik Numonov²  Khayrulla Bobakulov¹  Muhammad Nasimullah Qureshi²  Haiqing Zhao²  Haji Akber Aisa² 

[1]Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, 100170 Tashkent, Uzbekistan

[2] E-Mail:

[3]Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China

[4] E-Mails:

关键词: Hypericum scabrum L.;    ethylacetate fraction;    column chromatography;    NMR;    ESI-MS;    antidiabetic activity;    antioxidant activity;    antimicrobial activity;   

DOI  :  10.3390/molecules200611257

来源: mdpi

【 摘 要 】

Phytochemical investigations of ethyl acetate-soluble part of the aerial part of Hypericum scabrum L. delivered eight pure phenolic compounds 1–8. The pure compounds were identified through physico-chemical, NMR (1D, 2D) and mass spectrometric studies as: 3-8′′-bisapigenin (1), quercetin (2), quercetin-3-O-α-l-arabinofuranoside (3), quercetin-3-O-α-l-rhamnoside (4), quercetin-3-O-β-d-glucopyranoside (5), quercetin-3-O-β-d-galactopyranoside (6), (−)-epicatechin (7), (+)-catechin (8). Total polyphenolic compounds and total flavonoids contents were determined in the extract as 0.107 mg∙mg⁻¹ and 0.023 mg∙mg⁻¹ of the dried extract, respectively. Antioxidant activity using DPPH free radical scavenging assay delivered very strong activity for compounds 2 and 5, 6 and crude extract 10. Protein tyrosine phosphatase 1B (PTP-1B) inhibition experiment of isolated compounds and crude extracts resulted in significant inhibition activity for samples 2, 7a, 8a, 11 and 12 with IC₅₀ values ranging from 1.57 to 2.91 µM. Antimicrobial activity of the pure compounds and extracts produced average results against Staphylococcus aureus, Escherichia coli and Candida albicans strains. From our literature survey, it appears that all pure compounds except 2 were isolated and reported for the first time in H. scabrum.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.