Characterisation of Structural Proteins from Chronic Bee Paralysis Virus (CBPV) Using Mass Spectrometry

Viruses

Aurore Chevin⁴  Bruno Coutard³  Philippe Blanchard⁴  Anne-Sophie Dabert-Gay¹  Magali Ribière-Chabert⁴  Richard Thiéry⁴  Elke Genersch² 

[1] Institute of Molecular and Cellular Pharmacology, IPMC, UMR6097 CNRS, 660 route des Lucioles, 06560 Valbonne, France; E-Mail:ANSES, Sophia-Antipolis Laboratory, Bee Diseases Unit, BP 111, 06902 Sophia Antipolis, France;;Aix-Marseille Université, CNRS, AFMB UMR 7257, 13288 Marseille, France; E-Mail:;ANSES, Sophia-Antipolis Laboratory, Bee Diseases Unit, BP 111, 06902 Sophia Antipolis, France; E-Mails:

关键词: chronic bee paralysis virus (CBPV);    nano-HPLC;    MALDI-TOF/TOF;    protein identification;    hypothetical structural protein (hSP);    predicted structural protein (pSP);    anti-pSP antibodies;   

DOI  :  10.3390/v7062774

来源: mdpi

【 摘 要 】

Chronic bee paralysis virus (CBPV) is the etiological agent of chronic paralysis, an infectious and contagious disease in adult honeybees. CBPV is a positive single-stranded RNA virus which contains two major viral RNA fragments. RNA 1 (3674 nt) and RNA 2 (2305 nt) encode three and four putative open reading frames (ORFs), respectively. RNA 1 is thought to encode the viral RNA-dependent RNA polymerase (RdRp) since the amino acid sequence derived from ORF 3 shares similarities with the RdRP of families Nodaviridae and Tombusviridae. The genomic organization of CBPV and in silico analyses have suggested that RNA 1 encodes non-structural proteins, while RNA 2 encodes structural proteins, which are probably encoded by ORFs 2 and 3. In this study, purified CBPV particles were used to characterize virion proteins by mass spectrometry. Several polypeptides corresponding to proteins encoded by ORF 2 and 3 on RNA 2 were detected. Their role in the formation of the viral capsid is discussed.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.