Modeling Viral Infectious Diseases and Development of Antiviral Therapies Using Human Induced Pluripotent Stem Cell-Derived Systems

Viruses

Marta Trevisan¹  Alessandro Sinigaglia³  Giovanna Desole¹  Alessandro Berto¹  Monia Pacenti²  Giorgio Palù¹  Luisa Barzon¹ 

[1] Department of Molecular Medicine, University of Padova, via A. Gabelli 63, 35121 Padova, Italy; E-Mails:;Microbiology and Virology Unit, Padova University Hospital, via Giustiniani 2, 35128 Padova, Italy; E-Mail:;Veneto Institute of Oncology IOV IRCCS, via Gattamelata 64, 35128 Padova, Italy; E-Mail:

关键词: human induced pluripotent stem cells;    viral infection;    patient-specific disease model;    genome editing;    genetic susceptibility;    antiviral resistance;    CRISPR/Cas9;    personalized therapy;    hepatitis C virus;    human immunodeficiency virus;   

DOI  :  10.3390/v7072800

来源: mdpi

【 摘 要 】

The recent biotechnology breakthrough of cell reprogramming and generation of induced pluripotent stem cells (iPSCs), which has revolutionized the approaches to study the mechanisms of human diseases and to test new drugs, can be exploited to generate patient-specific models for the investigation of host–pathogen interactions and to develop new antimicrobial and antiviral therapies. Applications of iPSC technology to the study of viral infections in humans have included in vitro modeling of viral infections of neural, liver, and cardiac cells; modeling of human genetic susceptibility to severe viral infectious diseases, such as encephalitis and severe influenza; genetic engineering and genome editing of patient-specific iPSC-derived cells to confer antiviral resistance.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.