Potential Mechanism of Action of 3′-Demethoxy-6-O-demethyl-isoguaiacin on Methicillin Resistant Staphylococcus aureus

Molecules

Juan Manuel J. Favela-Hernández¹  Aldo F. Clemente-Soto¹  Isa໚s Balderas-Renter໚¹  Elvira Garza-González²  Mar໚ del Rayo Camacho-Corona¹ 

[1] Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo León, Av. Universidad S/N Ciudad Universitaria, San Nicolás de los Garza, Nuevo León C.P. 66451, Mexico; E-Mails:;Servicio de Gastroenterología y Departamento de Patología Clínica, Hospital Universitario, Dr. José Eleuterio González. Madero y Aguirre Pequeño, Universidad Autónoma de Nuevo León, Mitras Centro, Monterrey, Nuevo León C.P. 64460, Mexico; E-Mail:

关键词: Larrea tridentata;    3′-demethoxy-6-O-demethylisoguaiacin;    methicillin-resistant Staphylococcus aureus;    microarrays;    real time PCR;    mode of action;   

DOI  :  10.3390/molecules200712450

来源: mdpi

【 摘 要 】

Bacterial infections represent one of the main threats to global public health. One of the major causative agents associated with high morbidity and mortality infections in hospitals worldwide is methicillin-resistant Staphylococcus aureus. Therefore, there is a need to develop new antibacterial agents to treat these infections, and natural products are a rich source of them. In previous studies, we reported that lignan 3′-demethoxy-6-O-demethylisoguaiacin, isolated and characterized from Larrea tridentate, showed the best activity towards methicillin-resistant S. aureus. Thus, the aim of this study was to determine the potential molecular mechanism of the antibacterial activity of 3′-demethoxy-6-O-demethylisoguaiacin against methicillin-resistant S. aureus using microarray technology. Results of microarray genome expression were validated by real-time polymerase chain reaction (RT-PCR). The genetic profile expression results showed that lignan 3′-demethoxy-6-O-demethylisoguaiacin had activity on cell membrane affecting proteins of the ATP-binding cassette (ABC) transport system causing bacteria death. This molecular mechanism is not present in any antibacterial commercial drug and could be a new target for the development of novel antibacterial agents.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.