Potential Effect of CD271 on Human Mesenchymal Stromal Cell Proliferation and Differentiation

International Journal of Molecular Sciences

Giovanna Calabrese³  Raffaella Giuffrida³  Debora Lo Furno¹  Nunziatina Laura Parrinello²  Stefano Forte³  Rosario Gulino³  Cristina Colarossi²  Luciana Rita Schinocca²  Rosario Giuffrida¹  Venera Cardile¹  Lorenzo Memeo³ 

[1] Department of Biomedical and Biotechnological Sciences, Physiology Section, University of Catania, 95125 Catania, Italy; E-Mails:;Department of Experimental Oncology, Mediterranean Institute of Oncology, 95029 Viagrande, Italy; E-Mails:;IOM Ricerca, 95029 Viagrande, Italy; E-Mails:

关键词: mesenchymal stromal cells;    bone marrow;    adipose tissue;    adipogenic differentiation;    chondrogenic differentiation;    osteogenic differentiation;    CD271;   

DOI  :  10.3390/ijms160715609

来源: mdpi

【 摘 要 】

The Low-Affinity Nerve Growth Factor Receptor (LNGFR), also known as CD271, is a member of the tumor necrosis factor receptor superfamily. The CD271 cell surface marker defines a subset of multipotential mesenchymal stromal cells and may be used to isolate and enrich cells derived from bone marrow aspirate. In this study, we compare the proliferative and differentiation potentials of CD271⁺ and CD271⁻ mesenchymal stromal cells. Mesenchymal stromal cells were isolated from bone marrow aspirate and adipose tissue by plastic adherence and positive selection. The proliferation and differentiation potentials of CD271⁺ and CD271⁻ mesenchymal stromal cells were assessed by inducing osteogenic, adipogenic and chondrogenic in vitro differentiation. Compared to CD271⁺, CD271⁻ mesenchymal stromal cells showed a lower proliferation rate and a decreased ability to give rise to osteocytes, adipocytes and chondrocytes. Furthermore, we observed that CD271⁺ mesenchymal stromal cells isolated from adipose tissue displayed a higher efficiency of proliferation and trilineage differentiation compared to CD271⁺ mesenchymal stromal cells isolated from bone marrow samples, although the CD271 expression levels were comparable. In conclusion, these data show that both the presence of CD271 antigen and the source of mesenchymal stromal cells represent important factors in determining the ability of the cells to proliferate and differentiate.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.