Participation of S. Typhimurium cysJIH Operon in the H2S-mediated Ciprofloxacin Resistance in Presence of Sulfate as Sulfur Source

Antibiotics

Ricardo Álvarez²  Jorge Frávega²  Paula I. Rodas⁴  Juan A. Fuentes³  Daniel Paredes-Sabja¹  Iván L. Calderón²  Fernando Gil² 

[1] Gut Microbiota and Clostridia Research Group, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andres Bello, República 217, Santiago 837014, Chile; E-Mail:;Laboratorio de Genética y Patogénesis Bacteriana, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andres Bello, República 217, Santiago 8370146, Chile; E-Mails:;Laboratorio de Microbiología, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andres Bello, República 217, Santiago 8370146, Chile; E-Mail:;Center for Integrative Medicine and Innovative Science, Facultad de Medicina, Universidad Andres Bello, Echaurren 183, Santiago 8370071, Chile; E-Mail:

关键词: cysJIH;    ciprofloxacin resistance;    H₂S production;    sulfate;    sulfur source;   

DOI  :  10.3390/antibiotics4030321

来源: mdpi

【 摘 要 】

H₂S production has been proposed as a mechanism to explain bacterial resistance to antibiotics. In this work, we present evidence for the role of the cysJIH operon in resistance to ciprofloxacin mediated by H₂S production with different sulfate as the only sulfur source. We found that the products of the cysJIH operon are involved in ciprofloxacin resistance by increasing both, the levels of H₂S and reduced thiols apparently counteracting antimicrobial-induced reactive oxygen species (ROS). This protective effect was observed only when bacteria were cultured in the presence of sulfate, but not with cysteine, as the sole sulfur source.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.