Get to Understand More from Single-Cells: Current Studies of Microfluidic-Based Techniques for Single-Cell Analysis

International Journal of Molecular Sciences

Shih-Jie Lo²  Da-Jeng Yao¹ 

[1] Institute of Nanoengineering and Microsystem, National Tsing Hua University, Hsinchu 300, Taiwan; E-Mail

关键词: microfluidic techniques;    single-cell manipulation;    single-cell analysis;   

DOI  :  10.3390/ijms160816763

来源: mdpi

【 摘 要 】

This review describes the microfluidic techniques developed for the analysis of a single cell. The characteristics of microfluidic (e.g., little sample amount required, high-throughput performance) make this tool suitable to answer and to solve biological questions of interest about a single cell. This review aims to introduce microfluidic related techniques for the isolation, trapping and manipulation of a single cell. The major approaches for detection in single-cell analysis are introduced; the applications of single-cell analysis are then summarized. The review concludes with discussions of the future directions and opportunities of microfluidic systems applied in analysis of a single cell.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.