Preliminary Evaluation of Enteric Viruses in Bottled Mineral Water Commercialized in Brazil

Beverages

Vivian Rodrigues dos Santos²  Caroline Rigotto¹  Rodrigo Staggemeier²  Andréia Dalla Vecchia²  Andréia Henzel²  Fernando Rosado Spilki² 

[1]Laboratório de Microbiologia Molecular, Instituto de Ciências da Saúde, Universidade Feevale, Prédio Vermelho 2° Andar, Sala 205 RS 239, n° 2755, Vila Nova, 93352-000, Novo Hamburgo, RS, Brazil

关键词: enteric viruses;    HAdV;    hEV;    GARV;    mineral water;   

DOI  :  10.3390/beverages1030140

来源: mdpi

【 摘 要 】

In Brazil, mineral water is widely consumed and as of yet there have been no studies done in the country that aim to detect enteric viruses in this water source. The aim of this study was to analyze the presence of the human adenovirus (HAdV), the human rotavirus genogroup A (GARV) and the human enterovirus (hEV) in mineral water samples from six different brands that are commercialized in southern Brazil, using molecular techniques and comparing the results with bacterial indicators. Samples of 1.5 L and 500 mL were analyzed for viruses through PCR and total and thermotolerant coliforms. Additionally, heterotrophic bacteria were assayed using a commercial kit. The most prevalent virus was adenovirus (32.5%) followed by rotavirus (25%) and enterovirus (17.5%). Total and thermotolerant coliforms were absent in all samples and only three samples out of the 60 analyzed presented heterotrophic bacteria contamination. We conclude that, following the example taken into consideration regarding the public supply of drinking water, stricter measures for microbiological control should also be applied to mineral water so that this actually becomes a safer alternative.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.