Cold-Active, Heterotrophic Bacteria from the Highly Oligotrophic Waters of Lake Vanda, Antarctica

Microorganisms

Nicole A. Vander Schaaf¹  Anna M. G. Cunningham¹  Brandon P. Cluff³  CodyJo K. Kraemer³  Chelsea L. Reeves¹  Carli J. Riester¹  Lauren K. Slater¹  Michael T. Madigan⁴  W. Matthew Sattley¹  Ricardo Amils² 

[1] Division of Natural Sciences, Indiana Wesleyan University, 4201 South Washington St., Marion, IN 46953-4974, USA; E-Mails:Division of Natural Sciences, Indiana Wesleyan University, 4201 South Washington St., Marion, IN 46953-4974, USA;;Department of Biology, MidAmerica Nazarene University, 2030 E. College Way, Olathe, KS 66062, USA; E-Mails:;Department of Microbiology, Southern Illinois University, 1125 Lincoln Dr., Carbondale, IL 62901, USA; E-Mail:

关键词: Antarctica;    Lake Vanda;    McMurdo Dry Valleys;    psychrotolerant;    halotolerant;   

DOI  :  10.3390/microorganisms3030391

来源: mdpi

【 摘 要 】

The permanently ice-covered lakes of the McMurdo Dry Valleys, Antarctica are distinctive ecosystems that consist strictly of microbial communities. In this study, water samples were collected from Lake Vanda, a stratified Dry Valley lake whose upper waters (from just below the ice cover to nearly 60 m) are highly oligotrophic, and used to establish enrichment cultures. Six strains of psychrotolerant, heterotrophic bacteria were isolated from lake water samples from a depth of 50 or 55 m. Phylogenetic analyses showed the Lake Vanda strains to be species of Nocardiaceae, Caulobacteraceae, Sphingomonadaceae, and Bradyrhizobiaceae. All Lake Vanda strains grew at temperatures near or below 0 °C, but optimal growth occurred from 18 to 24 °C. Some strains showed significant halotolerance, but no strains required NaCl for growth. The isolates described herein include cold-active species not previously reported from Dry Valley lakes, and their physiological and phylogenetic characterization broadens our understanding of these limnologically unique lakes.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.