Determination of Ochratoxin A in Wheat and Maize by Solid Bar Microextraction with Liquid Chromatography and Fluorescence Detection

Toxins

Nabil Al-Hadithi²  Philip Kössler¹  Petr Karlovsky¹ 

[1] Molecular Phytopathology and Mycotoxin Research, Georg-August-University Göttingen, Grisebachstrasse 6, Göttingen 37077, Germany; E-Mails:;Faculty of Pharmaceutical Sciences, Hashemite University, Zarqa 13115, Jordan

关键词: ochratoxin A;    solid phase microextraction (SPME);    solid bar microextraction (SBME);   

DOI  :  10.3390/toxins7083000

来源: mdpi

【 摘 要 】

Solid bar microextraction (SBME), followed by liquid chromatography with fluorescence detection (HPLC-FLD), for the quantification of ochratoxin A in wheat and maize was developed. Ground wheat and maize grains were extracted with acetonitrile-water-acetic acid (79:20:1, v/v/v), followed by defatting with cyclohexane, and subjected to SBME-LC-FLD analysis. SBME devices were constructed by packing 2 mg sorbent (C18) into porous polypropylene micro-tubes (2.5 cm length, 600 μm i.d., and 0.2 μm pore size). SBME devices were conditioned with methanol and placed into 5 mL stirred sample solutions for 70 min. After extraction, OTA was desorbed into 200 μL of methanol for 15 min, the solution was removed in vacuum, the residue was dissolved in 50 μL of methanol-water (1:1, v/v) and ochratoxin A content was determined by HPLC-FLD. Under optimized extraction conditions, the limit of detection of 0.9 μg·kg⁻¹ and 2.5 μg·kg⁻¹ and the precision of 3.4% and 5.0% over a concentration range of 1 to 100 μg·kg⁻¹ in wheat and maize flour, respectively, were obtained.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.