Raman Spectroscopy of Optically Trapped Single Biological Micro-Particles

Sensors

Brandon Redding²  Mark J. Schwab²  Yong-le Pan¹ 

[1] U.S. Army Research Laboratory, 2800 Powder Mill Road, Adelphi, MD 20783, USA;

关键词: optical trapping;    Raman spectroscopy;    bioaerosols;   

DOI  :  10.3390/s150819021

来源: mdpi

【 摘 要 】

The combination of optical trapping with Raman spectroscopy provides a powerful method for the study, characterization, and identification of biological micro-particles. In essence, optical trapping helps to overcome the limitation imposed by the relative inefficiency of the Raman scattering process. This allows Raman spectroscopy to be applied to individual biological particles in air and in liquid, providing the potential for particle identification with high specificity, longitudinal studies of changes in particle composition, and characterization of the heterogeneity of individual particles in a population. In this review, we introduce the techniques used to integrate Raman spectroscopy with optical trapping in order to study individual biological particles in liquid and air. We then provide an overview of some of the most promising applications of this technique, highlighting the unique types of measurements enabled by the combination of Raman spectroscopy with optical trapping. Finally, we present a brief discussion of future research directions in the field.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.