Mapping of Powdery Mildew Resistance Gene pmCH89 in a Putative Wheat-Thinopyrum intermedium Introgression Line

International Journal of Molecular Sciences

Liyuan Hou¹  Xiaojun Zhang²  Xin Li²  Juqing Jia³  Huizhen Yang²  Haixian Zhan²  Linyi Qiao²  Huijuan Guo²  Zhijian Chang² 

[1] College of Life Science, Shanxi University, Taiyuan 030006, Shanxi, China; E-Mail:;Institute of Crop Science, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, Shanxi, China; E-Mails:;College of Agronomy, Shanxi Agricultural University, Taigu 030801, Shanxi, China; E-Mail:

关键词: wheat-Thinopyrum intermedium introgression line;    Th. intermedium;    powdery mildew;    genetic analysis;    molecular mapping;   

DOI  :  10.3390/ijms160817231

来源: mdpi

【 摘 要 】

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a globally serious disease adversely affecting wheat production. The Bgt-resistant wheat breeding line CH09W89 was derived after backcrossing a Bgt resistant wheat-Thinopyrum intermedium partial amphiploid TAI7045 with susceptible wheat cultivars. At the seedling stage, CH09W89 exhibited immunity or high resistance to Bgt pathotypes E09, E20, E21, E23, E26, Bg1, and Bg2, similar to its donor line TAI7045 and Th. intermedium. No Th. intermedium chromatin was detected based on genomic in situ hybridization of mitotic chromosomes. To determine the mode of inheritance of the Bgt resistance and the chromosomal location of the resistance gene, CH09W89 was crossed with two susceptible wheat cultivars. The results of the genetic analysis showed that the adult resistance to Bgt E09in CH09W89 was controlled by a single recessive gene, which was tentatively designated as pmCH89. Two polymorphic SSR markers, Xwmc310 and Xwmc125, were linked to the resistance gene with genetic distances 3.1 and 2.7 cM, respectively. Using the Chinese Spring aneuploid and deletion lines, the resistance gene and its linked markers were assigned to chromosome arm 4BL in the bin 0.68–0.78. Due to its unique position on chromosome 4BL, pmCH89 appears to be a new locus for resistance to powdery mildew. These results will be of benefit for improving powdery mildew resistance in wheat breeding programs.

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