Biosensors | |
Design and Characterization of a Sensorized Microfluidic Cell-Culture System with Electro-Thermal Micro-Pumps and Sensors for Cell Adhesion, Oxygen, and pH on a Glass Chip | |
Sebastian M. Bonk2  Marco Stubbe2  Sebastian M. Buehler1  Carsten Tautorat2  Werner Baumann2  Ernst-Dieter Klinkenberg3  Jan Gimsa2  | |
[1] Leibniz Institute for Farm Animal Biology, Institute of Muscle Biology and Growth, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany; E-Mail:;Chair for Biophysics, Department of Biology, University of Rostock, Gertrudenstr. 11a, 18057 Rostock, Germany; E-Mails:;DOT GmbH, Charles-Darwin-Ring 1A, 18059 Rostock, Germany; E-Mail: | |
关键词: lab-on chip; bone cells; thin film platinum sensors; acidification; respiration; | |
DOI : 10.3390/bios5030513 | |
来源: mdpi | |
【 摘 要 】
We combined a multi-sensor glass-chip with a microfluidic channel grid for the characterization of cellular behavior. The grid was imprinted in poly-dimethyl-siloxane. Mouse-embryonal/fetal calvaria fibroblasts (MC3T3-E1) were used as a model system. Thin-film platinum (Pt) sensors for respiration (amperometric oxygen electrode), acidification (potentiometric pH electrodes) and cell adhesion (interdigitated-electrodes structures, IDES) allowed us to monitor cell-physiological parameters as well as the cell-spreading behavior. Two on-chip electro-thermal micro-pumps (ETμPs) permitted the induction of medium flow in the system, e.g., for medium mixing and drug delivery. The glass-wafer technology ensured the microscopic observability of the on-chip cell culture. Connecting Pt structures were passivated by a 1.2 μm layer of silicon nitride (Si3N4). Thin Si3N4 layers (20 nm or 60 nm) were used as the sensitive material of the pH electrodes. These electrodes showed a linear behavior in the pH range from 4 to 9, with a sensitivity of up to 39 mV per pH step. The oxygen sensors were circular Pt electrodes with a sensor area of 78.5 μm2. Their sensitivity was 100 pA per 1% oxygen increase in the range from 0% to 21% oxygen (air saturated). Two different IDES geometries with 30- and 50-μm finger spacings showed comparable sensitivities in detecting the proliferation rate of MC3T3 cells. These cells were cultured for 11 days
【 授权许可】
CC BY
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
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