The Marine-Derived Fungus Clonostachys rosea, Source of a Rare Conjugated 4-Me-6E,8E-hexadecadienoic Acid Reducing Viability of MCF-7 Breast Cancer Cells and Gene Expression of Lipogenic Enzymes

Marine Drugs

Ana Camila Dos Santos Dias¹  Nicolas Ruiz¹  Aurélie Couzinet-Mossion¹  Samuel Bertrand¹  Muriel Duflos²  Yves-François Pouchus¹  Gilles Barnathan¹  Hassan Nazih¹  Gaetane Wielgosz-Collin¹ 

[1] Faculty of Pharmacy, University of Nantes, MMS, 9, Rue Bias, 44000 Nantes, France; E-Mails:;Faculty of Pharmacy, University of Nantes, IICiMed, 9 Rue Bias, 44000 Nantes, France; E-Mail:

关键词: Clonostachys rosea;    fatty acids;    conjugated fatty acid;    lipids;    cancer;    anti-carcinogenic;    MCF-7;    lipogenesis;    acetyl-CoA carboxylase;    fatty acid synthase;   

DOI  :  10.3390/md13084934

来源: mdpi

【 摘 要 】

A marine-derived strain of Clonostachys rosea isolated from sediments of the river Loire estuary (France) was investigated for its high lipid production. The fungal strain was grown on six different culture media to explore lipid production changes. An original branched conjugated fatty acid, mainly present in triglycerides and mostly produced when grown on DCA (23% of total fatty acid composition). It was identified as 4-Me-6E,8E-hexadecadienoic on the basis of spectroscopic analyses. This fatty acid reduced viability of MCF-7 breast cancer cells in a dose dependent manner (up to 63%) at physiological free fatty acid human plasma concentration (100 μM). Reduction of gene expression of two lipogenic enzymes, the acetyl CoA carboxylase (ACC) and the fatty acid synthase (FAS) was evaluated to explore the mechanisms of action of 4-Me-6E,8E-16:2 acid. At 50 μM, 50% and 35% of mRNA gene expression inhibition were observed for ACC and FAS, respectively.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.