Highly Sensitive Electrochemical Determination of Alfatoxin B1 Using Quantum Dots-Assembled Amplification Labels

Sensors

Xiaoqun Zeng¹  Huiju Gao¹  Daodong Pan¹  Yangying Sun¹  Jinxuan Cao¹  Zhen Wu¹  Zhenyu Pan² 

[1] Key Laboratory of Animal Protein Food Deep Processing Technology of Zhejiang, Ningbo University, Ningbo 315211, China; E-Mails:;School of Electronic Engineering, Xidian University, Xi’an 710071, China; E-Mail:

关键词: quantum dots;    layer-by-layer assembly;    electrochemical immunoassay;    AFB1 detection;   

DOI  :  10.3390/s150820648

来源: mdpi

【 摘 要 】

A competitive electrochemical immunoassay for highly sensitive detection of AFB1 is demonstrated using layer-by-layer (LBL) assembled quantum dots (QDs) as labels. To investigate the effects of the higher sensitivity of square wave voltammetric stripping (SWV) and of the LBL technique on the proposed immunoassays, the proposed assay was compared to electrochemical (EC) and fluorescent immunoassays, which did not use LBL technology. Peanut samples were analyzed using the three immunoassays. The limits of detection (LODs) were 0.018, 0.046 and 0.212 ng/mL, respectively, while the sensitivities were 0.308, 1.011 and 4.594 ng/mL, respectively. The proposed electrochemical immunoassay displayed a significant improvement in sensitivity, thereby providing a simple and sensitive alternative strategy for determining AFB1 levels in peanut samples.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.