Isolation and Characterization of Nano-Hydroxyapatite from Salmon Fish Bone

Materials

Jayachandran Venkatesan¹  Baboucarr Lowe⁴  Panchanathan Manivasagan¹  Kyong-Hwa Kang¹  Elna P. Chalisserry³  Sukumaran Anil²  Dong Gyu Kim⁵  Se-Kwon Kim¹ 

[1] Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Korea; E-Mails:;Dental Biomaterials Research Chair, King Saud University, Riyadh 12372, Saudi Arabia; E-Mail:;Stem Cell Unit, Department of Anatomy, King Saud University, Riyadh 12372, Saudi Arabia; E-Mail:;Department of Marine-bio Convergence Science, Pukyong National University, Busan 608-737, Korea; E-Mail:;Specialized Graduate School Science & Technology Convergence, Pukyong National University, Busan 608-739, Korea

关键词: hydroxyapatite;    salmon;    fish;    alkaline hydrolysis;   

DOI  :  10.3390/ma8085253

来源: mdpi

【 摘 要 】

Nano-Hydroxyapatite (nHA) was isolated from salmon bone by alkaline hydrolysis. The resulting nHA was characterized using several analytical tools, including thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction analysis (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM), to determine the purity of the nHA sample. The removal of organic matter from the raw fish was confirmed by TGA. FT-IR confirmed the presence of a carbonated group and the similarities to synthetic Sigma HA. XRD revealed that the isolated nHA was amorphous. Microscopy demonstrated that the isolated nHA possessed a nanostructure with a size range of 6–37 nm. The obtained nHA interacted with mesenchymal stem cells (MSCs) and was non-toxic. Increased mineralization was observed for nHA treated MSCs compared to the control group. These results suggest that nHA derived from salmon is a promising biomaterial in the field of bone tissue engineering.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.