Comparative Analysis of the Brassica napus Root and Leaf Transcript Profiling in Response to Drought Stress

International Journal of Molecular Sciences

Chunqing Liu¹  Xuekun Zhang²  Ka Zhang¹  Hong An¹  Kaining Hu¹  Jing Wen¹  Jinxiong Shen¹  Chaozhi Ma¹  Bin Yi¹  Jinxing Tu¹  Tingdong Fu¹ 

[1] National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070, China; E-Mails:;Key Laboratory of Oil Crop Biology and Genetic Breeding of the Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agriculture Sciences, Wuhan 430062, China; E-Mail:

关键词: Brassica napus;    drought stress;    root;    leaf;    transcriptome;    differentially expressed gene;   

DOI  :  10.3390/ijms160818752

来源: mdpi

【 摘 要 】

Drought stress is one of the major abiotic factors affecting Brassica napus (B. napus) productivity. In order to identify genes of potential importance to drought stress and obtain a deeper understanding of the molecular mechanisms regarding the responses of B. napus to dehydration stress, we performed large-scale transcriptome sequencing of B. napus plants under dehydration stress using the Illumina sequencing technology. In this work, a relatively drought tolerant B. napus line, Q2, identified in our previous study, was used. Four cDNA libraries constructed from mRNAs of control and dehydration-treated root and leaf were sequenced by Illumina technology. A total of 6018 and 5377 differentially expressed genes (DEGs) were identified in root and leaf. In addition, 1745 genes exhibited a coordinated expression profile between the two tissues under drought stress, 1289 (approximately 74%) of which showed an inverse relationship, demonstrating different regulation patterns between the root and leaf. The gene ontology (GO) enrichment test indicated that up-regulated genes in root were mostly involved in “stimulus” “stress” biological process, and activated genes in leaf mainly functioned in “cell” “cell part” components. Furthermore, a comparative network related to plant hormone signal transduction and AREB/ABF, AP2/EREBP, NAC, WRKY and MYC/MYB transcription factors (TFs) provided a view of different stress tolerance mechanisms between root and leaf. Some of the DEGs identified may be candidates for future research aimed at detecting drought-responsive genes and will be useful for understanding the molecular mechanisms of drought tolerance in root and leaf of B. napus.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.