Comparison of Cell Disruption Methods for Improving Lipid Extraction from Thraustochytrid Strains

Marine Drugs

Avinesh R. Byreddy²  Adarsha Gupta²  Colin J. Barrow²  Munish Puri¹ 

[1] Centre for Chemistry and Biotechnology, Geelong Technology Precinct, Deakin University, Waurn Ponds, Geelong 3217, Australia;

关键词: solvents;    biodiesel;    algae;    DHA;    marine;    omega-3;   

DOI  :  10.3390/md13085111

来源: mdpi

【 摘 要 】

Lipid extraction is an integral part of biodiesel production, as it facilitates the release of fatty acids from algal cells. To utilise thraustochytrids as a potential source for lipid production. We evaluated the extraction efficiency of various solvents and solvent combinations for lipid extraction from Schizochytrium sp. S31 and Thraustochytrium sp. AMCQS5-5. The maximum lipid extraction yield was 22% using a chloroform:methanol ratio of 2:1. We compared various cell disruption methods to improve lipid extraction yields, including grinding with liquid nitrogen, bead vortexing, osmotic shock, water bath, sonication and shake mill. The highest lipid extraction yields were obtained using osmotic shock and 48.7% from Schizochytrium sp. S31 and 29.1% from Thraustochytrium sp. AMCQS5-5. Saturated and monounsaturated fatty acid contents were more than 60% in Schizochytrium sp. S31 which suggests their suitability for biodiesel production.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.