An Investigation of the Cytotoxicity and Caspase-Mediated Apoptotic Effect of Green Synthesized Zinc Oxide Nanoparticles Using Eclipta prostrata on Human Liver Carcinoma Cells

Nanomaterials

Ill-Min Chung¹  Abdul Abdul Rahuman²  Sampath Marimuthu²  Arivarasan Vishnu Kirthi²  Karunanithi Anbarasan²  Govindasamy Rajakumar¹ 

[1] Department of Applied Bioscience, College of Life and Environmental Science, Konkuk University, Seoul 05029, Korea; E-Mail:;Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam 632509, Vellore District, Tamil Nadu, India; E-Mails:

关键词: anticancer actions;    caspase study;    zinc oxide nanoparticles;    Eclipta prostrata;   

DOI  :  10.3390/nano5031317

来源: mdpi

【 摘 要 】

Cancer is a leading cause of death worldwide and sustained focus is on the discovery and development of newer and better tolerated anticancer drugs, especially from plants. In the present study, a simple, eco-friendly, and inexpensive approach was followed for the synthesis of zinc oxide nanoparticles (ZnO NPs) using the aqueous leaf extract of Eclipta prostrata. The synthesized ZnO NPs were characterized by UV-visible absorption spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX), High-resolution transmission electron microscopy (HRTEM), and Selected area (electron) diffraction (SAED). The HRTEM images confirmed the presence of triangle, radial, hexagonal, rod, and rectangle, shaped with an average size of 29 ± 1.3 nm. The functional groups for synthesized ZnO NPs were 3852 cm⁻¹ for H-H weak peak, 3138 cm⁻¹ for aromatic C-H extend, and 1648 cm⁻¹ for Aromatic ring stretch. The 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT), caspase and DNA fragmentation assays were carried out using various concentrations of ZnO NPs ranging from 1 to 100 mg/mL. The synthesized ZnO NPs showed dose dependent cytopathic effects in the Hep-G2 cell line. At 100 mg/mL concentration, the synthesized ZnO NPs exhibited significant cytotoxic effects and the apoptotic features were confirmed through caspase-3 activation and DNA fragmentation assays.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.