期刊论文详细信息
International Journal of Environmental Research and Public Health
Oxidative Stress Mechanisms Caused by Ag Nanoparticles (NM300K) are Different from Those of AgNO3: Effects in the Soil Invertebrate Enchytraeus crypticus
Maria J. Ribeiro2  Vera L. Maria2  Janeck J. Scott-Fordsmand1  Mónica J. B. Amorim2 
[1] Department of Bioscience, Aarhus University, Vejlsovej 25, Silkeborg DK-8600, Denmark; E-Mail:;Department of Biology & CESAM, University of Aveiro, Aveiro 3810-193, Portugal; E-Mails:
关键词: antioxidant system;    reactive oxygen species;    metallothionein;    lipid damage;    soil;   
DOI  :  10.3390/ijerph120809589
来源: mdpi
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【 摘 要 】

The mechanisms of toxicity of Ag nanoparticles (NPs) are unclear, in particular in the terrestrial environment. In this study the effects of AgNP (AgNM300K) were assessed in terms of oxidative stress in the soil worm Enchytraeus crypticus, using a range of biochemical markers [catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione reductase (GR), total glutathione (TG), metallothionein (MT), lipid peroxidation (LPO)]. E. crypticus were exposed during 3 and 7 days (d) to the reproduction EC20, EC50 and EC80 levels of both AgNP and AgNO3. AgNO3 induced oxidative stress earlier (3 d) than AgNP (7 d), both leading to LPO despite the activation of the anti-redox system. MT increased only for AgNP. The Correspondence Analysis showed a clear separation between AgNO3 and AgNP, with e.g., CAT being the main descriptor for AgNP for 7 d. LPO, GST and GPx were for both 3 and 7 d associated with AgNO3, whereas MT and TG were associated with AgNP. These results may reflect a delay in the effects of AgNP compared to AgNO3 due to the slower release of Ag+ ions from the AgNP, although this does not fully explain the observed differences, i.e., we can conclude that there is a nanoparticle effect.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.

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