Influence of Different Three-Dimensional Open Porous Titanium Scaffold Designs on Human Osteoblasts Behavior in Static and Dynamic Cell Investigations

Materials

Jana Markhoff²  Jan Wieding²  Volker Weissmann¹  Juliane Pasold²  Anika Jonitz-Heincke²  Rainer Bader² 

[1] Institute for Polymer Technology, Alter Holzhafen 19, Wismar 23966, Germany; E-Mail:;University Medicine Rostock, Department of Orthopaedics, Biomechanics and Implant Technology Laboratory, Doberaner Strasse 142, Rostock 18057, Germany; E-Mails:

关键词: scaffold;    Ti-6Al-4V;    microstructure;    bioreactor;    human osteoblasts;    static culture;    dynamic culture;    additive manufacturing;   

DOI  :  10.3390/ma8085259

来源: mdpi

【 摘 要 】

In the treatment of osseous defects micro-structured three-dimensional materials for bone replacement serve as leading structure for cell migration, proliferation and bone formation. The scaffold design and culture conditions are crucial for the limited diffusion distance of nutrients and oxygen. In static culture, decreased cell activity and irregular distribution occur within the scaffold. Dynamic conditions entail physical stimulation and constant medium perfusion imitating physiological nutrient supply and metabolite disposal. Therefore, we investigated the influence of different scaffold configurations and cultivation methods on human osteoblasts. Cells were seeded on three-dimensional porous Ti-6Al-4V scaffolds manufactured with selective laser melting (SLM) or electron beam melting (EBM) varying in porosity, pore size and basic structure (cubic, diagonal, pyramidal) and cultured under static and dynamic conditions. Cell viability, migration and matrix production were examined via mitochondrial activity assay, fluorescence staining and ELISA. All scaffolds showed an increasing cell activity and matrix production under static conditions over time. Expectations about the dynamic culture were only partially fulfilled, since it enabled proliferation alike the static one and enhanced cell migration. Overall, the SLM manufactured scaffold with the highest porosity, small pore size and pyramidal basic structure proved to be the most suitable structure for cell proliferation and migration.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.