Antigenotoxicity and Tumor Growing Inhibition by Leafy Brassica carinata and Sinigrin

Molecules

Mar໚-Dolores Lozano-Baena³  Inmaculada Tasset¹  Sara Obregón-Cano³  Antonio de Haro-Bailon³  Andrés Muñoz-Serrano²  Ángeles Alonso-Moraga² 

[1] Department of Developmental and Molecular Biology, Chanin Building, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA; E-Mail:;Department of Genetics, Gregor Mendel Building, University of Córdoba, Rabanales, Córdoba 14014, Spain; E-Mails:;Department of Plant Breeding, Institute of Sustainable Agriculture, CSIC, Avd. Menendez Pidal s/n, Córdoba E-14004, Spain; E-Mails:

关键词: Drosophila melanogaster;    HL60;    antigenotoxicity;    cytotoxicity;    Brassica;    sinigrin;    bioactive compound;   

DOI  :  10.3390/molecules200915748

来源: mdpi

【 摘 要 】

Cruciferous vegetables are well known and worldwide consumed due to their health benefits and cancer prevention properties. As a desirable cruciferous plant, Ethiopian mustard (Brassica carinata A. Braun) and its glucosinolate sinigrin were tested in the in vivo Drosophila melanogaster (SMART) and the in vitro HL60 (human promyelocytic leukaemia cell line) systems. High performance liquid chromatography (HPLC) analysis of plant samples confirmed the presence of sinigrin as principal B. carinata glucosinolate. SMART was performed by feeding D. melanogaster larvae either with different concentrations of plant/compound samples or combining them with hydrogen peroxide (a potent oxidative mutagen) being both antimutagenics. HL60 assays showed the tumoricidal activity of plant samples (IC₅₀ = 0.28 mg·mL⁻¹) and the breakdown products of sinigrin hydrolysis (IC₅₀ = 2.71 µM). Our results enhance the potential of B. carinata as health promoter and chemopreventive in both systems and the leading role of sinigrin in these effects.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.