Feasibility of Primary Tumor Culture Models and Preclinical Prediction Assays for Head and Neck Cancer: A Narrative Review

Cancers

Amy J. C. Dohmen⁵  Justin E. Swartz²  Michiel W. M. Van Den Brekel⁵  Stefan M. Willems⁴  René Spijker¹  Jacques Neefjes³  Charlotte L. Zuur⁵ 

[1] Medical library, Academic Medical Center, Amsterdam 1100 DE, The Netherlands; E-Mail:;Department of Otorhinolaryngology—Head and Neck Surgery, University Medical Center Utrecht, Heidelberglaan 100, Utrecht 3508 GA, The Netherlands; E-Mail:;Department of Cell Biology, the Netherlands Cancer Institute—Antoni van Leeuwenhoek, Plesmanlaan 121, Amsterdam 1066 CX, The Netherlands; E-Mail:;Department of Pathology, University Medical Center Utrecht, Heidelberglaan 100, Utrecht 3508 GA, The Netherlands; E-Mail:;Department of Head and Neck Surgery and Oncology, The Netherlands Cancer Institute—Antoni van Leeuwenhoek, Plesmanlaan 121, Amsterdam 1066 CX, The Netherlands; E-Mails:

关键词: head neck cancer;    primary cell cultures;    chemosensitivity;    radiosensitivity;    personalized therapy;   

DOI  :  10.3390/cancers7030858

来源: mdpi

【 摘 要 】

Primary human tumor culture models allow for individualized drug sensitivity testing and are therefore a promising technique to achieve personalized treatment for cancer patients. This would especially be of interest for patients with advanced stage head and neck cancer. They are extensively treated with surgery, usually in combination with high-dose cisplatin chemoradiation. However, adding cisplatin to radiotherapy is associated with an increase in severe acute toxicity, while conferring only a minor overall survival benefit. Hence, there is a strong need for a preclinical model to identify patients that will respond to the intended treatment regimen and to test novel drugs. One of such models is the technique of culturing primary human tumor tissue. This review discusses the feasibility and success rate of existing primary head and neck tumor culturing techniques and their corresponding chemo- and radiosensitivity assays. A comprehensive literature search was performed and success factors for culturing in vitro are debated, together with the actual value of these models as preclinical prediction assay for individual patients. With this review, we aim to fill a gap in the understanding of primary culture models from head and neck tumors, with potential importance for other tumor types as well.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.