Rapid Bioassay-Guided Isolation of Antibacterial Clerodane Type Diterpenoid from Dodonaea viscosa (L.) Jaeq.

International Journal of Molecular Sciences

Muhammad Khurram¹  Linda A. Lawton²  Christine Edwards²  Marcello Iriti³  Abdul Hameed⁶  Murad A. Khan⁴  Farman A. Khan⁵  Shafiq ur Rahman¹ 

[1] Department of Pharmacy, Shaheed Benazir Bhutto University, Sheringal, Dir Upper 18000, Pakistan; E-Mail:;School of Pharmacy and Life Sciences, the Robert Gordon University, Aberdeen AB25 1HG, UK; E-Mails:;Department of Agricultural and Environmental Sciences, Milan State University, Milan 20133, Italy;Department of Chemistry, Kohat University of Science & Technology, Kohat 26000, Pakistan; E-Mail:;Department of Chemistry, Shaheed Benazir Bhutto University, Sheringal, Dir Upper 18000, Pakistan; E-Mail:;Department of Microbiology, Quaid-i-Azam University, Islamabad 45320, Pakistan; E-Mail:

关键词: Dodonaea viscosa;    preparative HPLC;    XTT assay;    clerodane diterpenoid;   

DOI  :  10.3390/ijms160920290

来源: mdpi

【 摘 要 】

Plant extracts are complex matrices and, although crude extracts are widely in use, purified compounds are pivotal in drug discovery. This study describes the application of automated preparative-HPLC combined with a rapid off-line bacterial bioassay, using reduction of a tetrazolium salt as an indicator of bacterial metabolism. This approach enabled the identification of fractions from Dodonaea viscosa that were active against Staphylococcus aureus and Escherichia coli, which, ultimately, resulted in the identification of a clerodane type diterpenoid, 6β-hydroxy-15,16-epoxy-5β, 8β, 9β, 10α-cleroda-3, 13(16), 14-trien-18-oic acid, showing bacteriostatic activity (minimum inhibitory concentration (MIC) = 64–128 µg/mL) against test bacteria. To the best of our knowledge, this is the first report on antibacterial activity of this metabolite from D. viscosa.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.