An Apta-Biosensor for Colon Cancer Diagnostics

Sensors

Mojgan Ahmadzadeh Raji⁴  Ghasem Amoabediny²  Parviz Tajik³  Morteza Hosseini⁴  Ebrahim Ghafar-Zadeh¹ 

[1] Department of Electrical Engineering and Computer Science, York University, Toronto, ON M3J1P3, Canada;Department of Biotechnology and Pharmacy Engineering, Faculty of Chemical Engineering, University of Tehran, Tehran 4563-11155, Iran;Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran 1419963111, Iran; E-Mail:;Department of Nanobiotechnology, School of New Sciences and Technologies, University of Tehran, Tehran14395-1561, Iran; E-Mails:

关键词: aptasensor;    aptamer;    cyclic voltammetry (CV);    colon cancer;    biosensor;    fluorescence labeling;   

DOI  :  10.3390/s150922291

来源: mdpi

【 摘 要 】

This paper reports the design and implementation of an aptasensor using a modified KCHA10a aptamer. This aptasensor consists of a functionalized electrodes using various materials including 11-mercaptoandecanoic acid (11-MUA) and modified KCHA10a aptamer. The HCT 116, HT 29 and HEp-2 cell lines are used in this study to demonstrate the functionality of aptasensor for colon cancer detection purposes. Flow cytometry, fluorescence microscopy and electrochemical cyclic voltammetry are used to verify the binding between the target cells and aptamer. The limit of detection (LOD) of this aptasensor is equal to seven cancer cells. Based on the experimental results, the proposed sensor can be employed for point-of-care cancer disease diagnostics.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.