MicroRNAs in Salivary Exosome as Potential Biomarkers of Aging

International Journal of Molecular Sciences

Tatsuya Machida³  Takaaki Tomofuji³  Daisuke Ekuni³  Takayuki Maruyama¹  Toshiki Yoneda³  Yuya Kawabata³  Hirofumi Mizuno³  Hisataka Miyai³  Muneyoshi Kunitomo³  Manabu Morita³  Nalini Santanam² 

[1] Center for Innovative Clinical Medicine, Okayama University Hospital, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan; E-Mail:Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan;;Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan; E-Mails:

关键词: microRNA;    saliva;    exosome;    aging;   

DOI  :  10.3390/ijms160921294

来源: mdpi

【 摘 要 】

The aim of this study was to examine whether salivary exosomal miRNAs could be identified as aging biomarkers. Fifteen young healthy volunteers (median age, 21.0 years) and 13 old individuals (median age, 66.0 years) were recruited. Unstimulated whole saliva was collected, salivary exosomes were isolated, and total RNA was extracted. In a microarray, 242 miRNAs were commonly detected in these two mixed samples. Based on the cut-off values of 2- or 0.5-fold changes (FC) and regulatory power for aging process, six candidate miRNAs (miR-24-3p, miR-371a-5p, miR-3175, miR-3162-5p, miR-671-5p, and miR-4667-5p) were selected. After comparing each total RNA obtained by the 15 young and 13 old individuals to validate the FC values using quantitative real-time PCR, miR-24-3p was identified as a novel candidate aging biomarker. This pilot study suggested that salivary exosomal miRNAs could be identified as candidate aging biomarkers. To confirm whether miR-24-3p in salivary exosomes are suitable biomarkers of aging, further validation research is required.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.