Constructing Biopolymer-Inorganic Nanocomposite through a Biomimetic Mineralization Process for Enzyme Immobilization

Materials

Jian Li²  Jun Ma²  Tao Jiang²  Yanhuan Wang²  Xuemei Wen¹  Guozhu Li³ 

[1] Tianjin Synthetic Material Research Institute, Tianjin 300220, China; E-Mail:;School of Material Science and Chemical Engineering, Tianjin University of Science and Technology, Tianjin 300457, China; E-Mails:;Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; E-Mail:

关键词: biomimetic mineralization;    nanocomposite;    enzyme immobilization;    silica;    alginate;    template;   

DOI  :  10.3390/ma8095286

来源: mdpi

【 摘 要 】

Inspired by biosilicification, biomimetic polymer-silica nanocomposite has aroused a lot of interest from the viewpoints of both scientific research and technological applications. In this study, a novel dual functional polymer, NH₂-Alginate, is synthesized through an oxidation-amination-reduction process. The “catalysis function” ensures the as-prepared NH₂-Alginate inducing biomimetic mineralization of silica from low concentration precursor (Na₂SiO₃), and the “template function” cause microscopic phase separation in aqueous solution. The diameter of resultant NH₂-Alginate micelles in aqueous solution distributed from 100 nm to 1.5 μm, and is influenced by the synthetic process of NH₂-Alginate. The size and morphology of obtained NH₂-Alginate/silica nanocomposite are correlated with the micelles. NH₂-Alginate/silica nanocomposite was subsequently utilized to immobilize β-Glucuronidase (GUS). The harsh condition tolerance and long-term storage stability of the immobilized GUS are notably improved due to the buffering effect of NH₂-Alginate and cage effect of silica matrix.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.