Development of a Rapid LC-MS/MS Method for the Determination of Emerging Fusarium mycotoxins Enniatins and Beauvericin in Human Biological Fluids

Toxins

Ana Belén Serrano¹  Anna Laura Capriotti²  Chiara Cavaliere²  Susy Piovesana²  Roberto Samperi²  Salvatore Ventura²  Aldo Laganಠ

[1] Laboratorio de Toxicología, Departament de Medicina Preventiva I Salut Pública, Ciències de l’Alimentació, Toxicologia I Medicina Legal Facultat de Farmàcia, Universitat de València, València 46010, Spain; E-Mail:;Department of Chemistry, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, Rome 00185, Italy; E-Mails:

关键词: mycotoxins;    enniatins;    beauvericin;    urine;    plasma;    liquid chromatography-tandem mass spectrometry;   

DOI  :  10.3390/toxins7093554

来源: mdpi

【 摘 要 】

A novel method for the simultaneous determination of enniatins A, A1, B and B1 and beauvericin, both in human urine and plasma samples, was developed and validated. The method consisted of a simple and easy pretreatment, specific for each matrix, followed by solid phase extraction (SPE) and detection by high performance liquid chromatography-tandem mass spectrometry with an electrospray ion source. The optimized SPE method was performed on graphitized carbon black cartridges after suitable dilution of the extracts, which allowed high mycotoxin absolute recoveries (76%–103%) and the removal of the major interferences from the matrix. The method was extensively evaluated for plasma and urine samples separately, providing satisfactory results in terms of linearity (R² of 0.991–0.999), process efficiency (>81%), trueness (recoveries between 85% and 120%), intra-day precision (relative standard deviation, RSD < 18%), inter-day precision (RSD < 21%) and method quantification limits (ranging between 20 ng·L⁻¹ and 40 ng·L⁻¹ in plasma and between 5 ng·L⁻¹ and 20 ng·L⁻¹ in urine). Finally, the highly sensitive validated method was applied to some urine and plasma samples from different donors.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.