Steady-State Fluorescence and Lifetime Emission Study of pH-Sensitive Probes Based on i-motif Forming Oligonucleotides Single and Double Labeled with Pyrene

Chemosensors

Anna Dembska¹  Patrycja Rzepecka²  Bernard Juskowiak² 

[1] Adam Mickiewicz University, Faculty of Chemistry, Umultowska 89b, 61-614 Poznan, Poland;

关键词: fluorescence;    lifetime;    pyrene;    excimer;    oligonucleotide probe;    i-motif;   

DOI  :  10.3390/chemosensors3030211

来源: mdpi

【 摘 要 】

Cytosine-rich nucleic acids undergo pH-stimulated structural transitions leading to formation of an i-motif architecture at an acidic pH. Thus, i-motifs are good foundation for designing simple pH-sensitive fluorescent probes. We report here steady-state and time-resolved fluorescence studies of pyrene-labeled probes based on RET sequence: C₄GC₄GC₄GC₄TA (RET21), AC₄GC₄GC₄GC₄TA (RET21A) and C₄GC₄GC₄GC₄T (RET20). Comparative studies with single- and double-labeled i-motif probes were carried out. For each probe, we have measured fluorescence spectra and decays for emission wavelength of 390 nm over a wide range of pH (from 4.0 to 8.0). Effect of the oligonucleotide sequence and the number of pyrene labels on the spectral characteristics of probes were discussed.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.