First Report of Ciguatoxins in Two Starfish Species: Ophidiaster ophidianus and Marthasterias glacialis

Toxins

Marisa Silva³  Inés Rodriguez⁴  Aldo Barreiro³  Manfred Kaufmann²  Ana Isabel Neto²  Meryem Hassouani¹  Brahim Sabour¹  Amparo Alfonso⁴  Luis M. Botana⁴  Vitor Vasconcelos³ 

[1] Phycology Research Unit—Biotechnology, Ecosystems Ecology and Valorization Laboratory. Faculty of Sciences El Jadida, University Chouaib Doukkali, El Jadida BP20, Morocco; E-Mails:;Interdisciplinary Center of Marine and Environmental Research—CIMAR/CIIMAR, University of Porto, Rua dos Bragas, 289, Porto 4050-123, Portugal; E-Mails:;Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, Porto 4619-007, Portugal; E-Mails:;Department of Pharmacology, Faculty of Veterinary, University of Santiago of Compostela, Lugo 27002, Spain; E-Mails:

关键词: ciguatera;    new vectors;    Madeira Island;    São Miguel Island;    Morocco;   

DOI  :  10.3390/toxins7093740

来源: mdpi

【 摘 要 】

Ciguatera fish poisoning (CFP) is a syndrome caused by the ingestion of fish contaminated with Ciguatoxins (CTXs). These phycotoxins are produced mainly by dinoflagellates that belong to the genus Gambierdiscus that are transformed in more toxic forms in predatory fish guts, and are more present in the Indo-Pacific and Caribbean areas. It is estimated that CFP causes per year more than 10,000 intoxications worldwide. With the rise of water temperature and anthropogenic intervention, it is important to study the prevalence of CFP in more temperate waters. Through inter- and subtidal sampling, 22 species of organisms were collected, in Madeira and Azores archipelagos and in the northwestern Moroccan coast, during September of 2012 and June and July of 2013. A total of 94 samples of 22 different species of bivalves, gastropods, echinoderms and crustaceans where analyzed by Ultra Performance Liquid Chromatography-Mass Spectometry-Ion Trap-Time of Flight (UPLC-MS-IT-TOF) and Ultra Performance Chromatography- Mass Spectrometry (UPLC-MS). Our main aim was to detect new vectors and ascertain if there were some geographical differences. We detected for the first time putative CTXs in echinoderms, in two starfish species—M. glacialis and O. ophidianus. We detected differences regarding uptake values by organisms and geographical location. Toxin amounts were significant, showing the importance and the need for continuity of these studies to gain more knowledge about the prevalence of these toxins, in order to better access human health risk. In addition, we suggest monitoring of these toxins should be extended to other vectors, starfish being a good alternative for protecting and accessing human health risk.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.