Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan

Toxins

Polina A. Kameneva¹  Kseniya V. Efimova¹  Viacheslav G. Rybin¹  Tatiana Y. Orlova¹ 

[1] A.V. Zhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo St., 17, Vladivostok 690041, Russia; E-Mails:

关键词: Prorocentrum foraminosum;    DTX-1;    molecular identification;    HPLC-HRMS;    Russia;   

DOI  :  10.3390/toxins7103947

来源: mdpi

【 摘 要 】

For the first time the presence of dinophysistoxin-1 (DTX-1) in a culture of Prorocentrum foraminosum was revealed in cells and in the culture medium. The clone was isolated from coastal waters of the Sea of Japan and identified by molecular analyses of SSU and D1/D2 regions of LSU rDNA. The concentration of DTX-1 in cells was 8.4 ± 2.5 pg/cell and, in cell-free media, 27.9 ± 14.7 µg/L. The toxin presence was confirmed by HPLC with high-resolution tandem mass-spectrometry.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.