Astaxanthin Inhibits Proliferation and Induces Apoptosis of Human Hepatocellular Carcinoma Cells via Inhibition of Nf-Κb P65 and Wnt/Β-Catenin in Vitro

Marine Drugs

Jingjing Li¹  Weiqi Dai¹  Yujing Xia¹  Kan Chen¹  Sainan Li¹  Tong Liu¹  Rong Zhang¹  Jianrong Wang¹  Wenxia Lu¹  Yuqing Zhou¹  Qin Yin¹  Huerxidan Abudumijiti¹  Rongxia Chen¹  Yuanyuan Zheng¹  Fan Wang¹  Jie Lu¹  Yingqun Zhou¹  Chuanyong Guo¹ 

[1] Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, China; E-Mails:

关键词: hepatocellular carcinoma;    astaxanthin;    apoptosis;   

DOI  :  10.3390/md13106064

来源: mdpi

【 摘 要 】

Hepatocellular carcinoma (HCC) is a malignant tumor that can cause systemic invasion; however, the exact etiology and molecular mechanism are unknown. Astaxanthin (ASX), a powerful antioxidant, has efficient anti-oxidant, anti-inflammatory, and other activities, and has great research prospects in cancer therapy. We selected the human hepatoma cell lines, LM3 and SMMC-7721, to study the anti-tumor effect and related mechanisms of ASX. The cell lines were treated with different concentrations of ASX, and its solvent DMSO as a control, for different time periods and the results were determined using CCK8, qRT-PCR, WB, apoptotic staining, and flow cytometry. ASX induced significant apoptosis of HCC cells, and its effect may have been caused by NF-κB p65 and Wnt/β-catenin down-regulation via negative activation of PI3K/Akt and ERK. Antitumor research on ASX has provided us with a potential therapy for patients with hepatomas.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.