Ionic Liquid-Based Ultrasonic-Assisted Extraction of Secoisolariciresinol Diglucoside from Flaxseed (Linum usitatissimum L.) with Further Purification by an Aqueous Two-Phase System

Molecules

Zhi-Jian Tan¹  Chao-Yun Wang¹  Zi-Zhen Yang²  Yong-Jian Yi¹  Hong-Ying Wang¹  Wan-Lai Zhou¹  Fen-Fang Li² 

[1] Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China; E-Mails:;College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China; E-Mails:

关键词: ionic liquid;    ultrasonic-assisted extraction;    aqueous two-phase system;    secoisolariciresinol diglucoside;    purification;   

DOI  :  10.3390/molecules201017929

来源: mdpi

【 摘 要 】

In this work, a two-step extraction methodology of ionic liquid-based ultrasonic-assisted extraction (IL-UAE) and ionic liquid-based aqueous two-phase system (IL-ATPS) was developed for the extraction and purification of secoisolariciresinol diglucoside (SDG) from flaxseed. In the IL-UAE step, several kinds of ILs were investigated as the extractants, to identify the IL that affords the optimum extraction yield. The extraction conditions such as IL concentration, ultrasonic irradiation time, and liquid–solid ratio were optimized using response surface methodology (RSM). In the IL-ATPS step, ATPS formed by adding kosmotropic salts to the IL extract was used for further separation and purification of SDG. The most influential parameters (type and concentration of salt, temperature, and pH) were investigated to obtain the optimum extraction efficiency. The maximum extraction efficiency was 93.35% under the optimal conditions of 45.86% (w/w) IL and 8.27% (w/w) Na₂SO₄ at 22 °C and pH 11.0. Thus, the combination of IL-UAE and IL-ATPS makes up a simple and effective methodology for the extraction and purification of SDG. This process is also expected to be highly useful for the extraction and purification of bioactive compounds from other important medicinal plants.

【 授权许可】

CC BY   
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