Isolation and Genome Characterization of the Virulent Staphylococcus aureus Bacteriophage SA97

Viruses

Yoonjee Chang¹  Hakdong Shin¹  Ju-Hoon Lee³  Chul Jong Park⁴  Soon-Young Paik²  Sangryeol Ryu¹ 

[1] Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, and Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, Korea;Department of Microbiology, College of Medicine, the Catholic University of Korea, Seoul 137-701, Korea;Department of Food Science and Biotechnology, Kyung Hee University, Yongin 446-701, Korea;Department of Dermatology, College of Medicine, the Catholic University of Korea, Seoul 137-701, Korea;

关键词: bacteriophage;    genome analysis;    Staphylococcus aureus;   

DOI  :  10.3390/v7102870

来源: mdpi

【 摘 要 】

A novel bacteriophage that infects S. aureus, SA97, was isolated and characterized. The phage SA97 belongs to the Siphoviridae family, and the cell wall teichoic acid (WTA) was found to be a host receptor of the phage SA97. Genome analysis revealed that SA97 contains 40,592 bp of DNA encoding 54 predicted open reading frames (ORFs), and none of these genes were related to virulence or drug resistance. Although a few genes associated with lysogen formation were detected in the phage SA97 genome, the phage SA97 produced neither lysogen nor transductant in S. aureus. These results suggest that the phage SA97 may be a promising candidate for controlling S. aureus.

【 授权许可】

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.