Preparation, Characterization and Evaluation of α-Tocopherol Succinate-Modified Dextran Micelles as Potential Drug Carriers

Materials

Jingmou Yu¹  Yunfeng Zhou¹  Wencong Chen¹  Jin Ren¹  Lifang Zhang¹  Lu Lu¹  Gan Luo¹  Hao Huang² 

[1] School of Pharmacy and Life Sciences, Jiujiang University, 320 Xunyang East Road, Jiujiang 332000, China;;School of Chemical and Biological Engneering, Yichun University, 576 Xuefu Road, Yichun 336000, China

关键词: dextran;    α-tocopherol succinate;    polymeric micelles;    doxorubicin;    drug carrier;   

DOI  :  10.3390/ma8105332

来源: mdpi

【 摘 要 】

In the present study, α-tocopherol succinate (TOS) conjugated dextran (Dex-TOS) was synthesized and characterized by fourier transform infrared (FT-IR) spectroscopy, ¹H nuclear magnetic resonance (¹H NMR), dynamic light scattering (DLS) and fluorescence spectroscopy. Dex-TOS could form nanoscaled micelles in aqueous medium. The critical micelle concentration (CMC) is 0.0034 mg/mL. Doxorubicin (Dox) was selected as a model drug. Dox-loaded Dex-TOS (Dex-TOS/Dox) micelles were prepared by a dialysis method. The size of Dex-TOS/Dox micelles increased from 295 to 325 nm with the Dox-loading content increasing from 4.21% to 8.12%. The Dex-TOS/Dox micelles were almost spherical in shape, as determined by transmission electron microscopy (TEM). In vitro release demonstrated that Dox release from the micelles was in a sustained manner for up to 96 h. The cellular uptake of Dex-TOS/Dox micelles in human nasopharyngeal epidermoid carcinoma (KB) cells is an endocytic process determined by confocal laser scanning microscopy (CLSM). Moreover, Dex-TOS/Dox micelles exhibited comparable cytotoxicity in contrast with doxorubicin hydrochloride. These results suggested that Dex-TOS micelles could be a promising carrier for drug delivery.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.