Cytotoxic Evaluation of e-Liquid Aerosol using Different Lung-Derived Cell Models

International Journal of Environmental Research and Public Health

Stefanie Scheffler¹  Hauke Dieken²  Olaf Krischenowski²  Michaela Aufderheide² 

[1] Cultex Laboratories GmbH, Feodor-Lynen-Street 21, 30625 Hannover, Germany;

关键词: electronic cigarette;    smoking;    tobacco;    nicotine;    cytotoxicity;    immortalized normal bronchial epithelial cells;    air-liquid interface;    CL-1548;    CULTEX^® RFS;    public health;   

DOI  :  10.3390/ijerph121012466

来源: mdpi

【 摘 要 】

The in vitro toxicological evaluation of e-liquid aerosol is an important aspect of consumer protection, but the cell model is of great significance. Due to its water solubility, e-liquid aerosol is deposited in the conducting zone of the respiratory tract. Therefore, primary normal human bronchial epithelial (NHBE) cells are more suitable for e-liquid aerosol testing than the widely used alveolar cell line A549. Due to their prolonged lifespan, immortalized cell lines derived from primary NHBE cells, exhibiting a comparable in vitro differentiation, might be an alternative for acute toxicity testing. In our study, A549 cells freshly isolated NHBE cells and the immortalized cell line CL-1548 were exposed at the air-liquid interface to e-liquid aerosol and cigarette mainstream smoke in a CULTEX^® RFS compact module. The cell viability was analyzed 24 h post-exposure. In comparison with primary NHBE cells, the CL-1548 cell line showed lower sensitivity to e-liquid aerosol but significantly higher sensitivity compared to A549 cells. Therefore, the immortalized cell line CL-1548 is recommended as a tool for the routine testing of e-liquid aerosol and is preferable to A549 cells.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.