Phytochemical and Biological Investigation of Two Diplotaxis Species Growing in Tunisia: D. virgata & D. erucoides

Molecules

Nizar Ben Salah²  Hervé Casabianca³  Hichem Ben Jannet¹  Sophie Chenavas³  Corinne Sanglar³  Aurélie Fildier³  Nabiha Bouzouita⁴ 

[1] Laboratoire de Chimie Hétérocyclique, Produits Naturels et Réactivité, Equipe, Chimie Médicinale et Produits Naturels, Faculté des Sciences de Monastir, Université de Monastir, Avenue of the Environment, 5019 Monastir, Tunisia; E-Mail:;Faculté des Sciences de Monastir, Université de Monastir, Avenue de l’Environnement, 5019 Monastir, Tunisia; E-Mail:;Département Service Central d’analyse, Institut des Sciences Analytiques, Université de Lyon, UMR 5280 CNRS, Université; Lyon 1, ENS-Lyon, 5 Rue de la Doua 69100 Villeurbanne, France; E-Mails:;Laboratoire de Chimie Organique Structurale, Synthèse et Etude Physicochimique–Faculté des Sciences de Tunis, 2092 El Manar, Tunisia

关键词: D. erucoides;    D. virgata;    flavonoids;    fatty acids;    NMR;    antioxidant activity;    antimicrobial activity;   

DOI  :  10.3390/molecules201018128

来源: mdpi

【 摘 要 】

A phytochemical investigation of Diplotaxis virgata D.C. and D. erucoides (L.) D.C. (Brassicaceae) offered to the isolation of two new flavonoids isorhamnetin-3-O-α-l-glucopyranoside (1) and rhamnetin-3,3ʹ-di-O-β-d-glucopyranoside (2), respectively. Their structures have been elucidated from the extended spectroscopic methods, including 1D- and 2D-NMR, UV and mass spectrometry analysis and by comparison with literature data. The fatty acid composition of the hexane extracts of the two species was also investigated by using GC-MS. The antioxidant activity of ethanol, ethyl acetate, n-butanol extracts and the isolated compounds from the two species was evaluated using DPPH and ABTS⁺ scavenging assays. All the tested samples showed an efficient radical scavenging ability, with IC₅₀ values ranging from 16–40 µg/mL for the DPPH and from 17–44 µg/mL for the ABTS⁺ assays. In addition, the antibacterial activity of the prepared extracts and compounds 1 and 2, determined by well diffusion agar method against two Gram positive and five Gram negative bacteria, was evaluated and the results showed significant effects against all strains used.

【 授权许可】

CC BY   
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