Two-Dimensional N-Glycan Distribution Mapping of Hepatocellular Carcinoma Tissues by MALDI-Imaging Mass Spectrometry

Biomolecules

Thomas W. Powers³  Stephanie Holst²  Manfred Wuhrer²  Anand S. Mehta¹  Richard R. Drake³ 

[1] Department of Microbiology and Immunology, College of Medicine, Drexel University, 2900 W. Queen Lane, Philadelphia, PA 19129, USA; E-Mail:;Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden 2333ZA, The Netherlands; E-Mails:;Department of Cell and Molecular Pharmacology and Experimental Therapeutics and MUSC Proteomics Center, Medical University of South Carolina, 173 Ashley Avenue, Charleston, SC 29425, USA; E-Mail:

关键词: N-linked glycosylation;    formalin-fixed paraffin-embedded tissue;    hepatocellular carcinoma;    MALDI imaging mass spectrometry;    glycoprotein;   

DOI  :  10.3390/biom5042554

来源: mdpi

【 摘 要 】

A new mass spectrometry imaging approach to simultaneously map the two-dimensional distribution of N-glycans in tissues has been recently developed. The method uses Matrix Assisted Laser Desorption Ionization Imaging Mass Spectrometry (MALDI-IMS) to spatially profile the location and distribution of multiple N-linked glycan species released by peptide N-glycosidase F in frozen or formalin-fixed tissues. Multiple formalin-fixed human hepatocellular carcinoma tissues were evaluated with this method, resulting in a panel of over 30 N-glycans detected. An ethylation reaction of extracted N-glycans released from adjacent slides was done to stabilize sialic acid containing glycans, and these structures were compared to N-glycans detected directly from tissue profiling. In addition, the distribution of singly fucosylated N-glycans detected in tumor tissue microarray cores were compared to the histochemistry staining pattern of a core fucose binding lectin. As this MALDI-IMS workflow has the potential to be applied to any formalin-fixed tissue block or tissue microarray, the advantages and limitations of the technique in context with other glycomic methods are also summarized.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.