Identification and Expression Analysis of Zebrafish (Danio rerio) E-Selectin during Embryonic Development

Molecules

Guijin Sun¹  Kechun Liu²  Xue Wang²  Xiuhe Liu¹  Qiuxia He²  Chung-Der Hsiao³ 

[1] School of Food Science and Engineering, Qilu University of Technology, Jinan 250014, China; E-Mails:;Biology Institute, Shandong Academy of Sciences, Jinan 250014, China; E-Mails:;Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li 32023, Taiwan; E-Mail:

关键词: zebrafish;    E-selectin;    identification;    expression;    lipopolysaccharide;   

DOI  :  10.3390/molecules201018539

来源: mdpi

【 摘 要 】

In this study, we cloned the full-length cDNA of E-selectin of zebrafish (Danio rerio), analyzed its expression pattern and preliminarily explored its biological function. Zebrafish E-selectin cDNA is 3146 bp and encodes a putative 871 amino acid protein. All structural domains involved in E-selectin function are conserved in the putative protein. Whole-mount in situ hybridization of zebrafish at 24 and 48 h post-fertilization (hpf) revealed E-selectin expression mainly in vascular/endothelial progenitor cells in the posterior trunk and blood cells in the intermediate cell mass and posterior cardinal vein regions. Real-time quantitative RT-PCR analysis detected E-selectin expression at 0.2, 24 and 48 hpf and significantly decreased from 48 to 72 hpf. The expression of E-selectin, tumor necrosis factor-α and interleukin-1β was significantly upregulated at 22 to 72 h after induction with bacterial lipopolysaccharide. Thus, the structure of E-selectin protein is highly conserved among species, and E-selectin may be involved in embryonic development and essential for hematopoiesis and angiogenesis during embryonic development in zebrafish. Furthermore, we provide the first evidence of inflammatory mediators inducing E-selectin expression in non-mammalian vertebrates, which suggests that zebrafish E-selectin may be involved in inflammation and probably has similar biological function to mammalian E-selectin.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.