Protein Adsorption in Microengraving Immunoassays

Sensors

Qing Song¹ 

[1] Chemical and Biomolecular Engineering, New York University Polytechnic School of Engineering, 6 Metro Tech Center, Brooklyn, NY 11201, USA; E-Mail

关键词: protein adsorption;    interface;    transport dynamics;    transport mechanisms;    single cells;    protein secretion;    microengraving;    immunoassay;   

DOI  :  10.3390/s151026236

来源: mdpi

【 摘 要 】

Microengraving is a novel immunoassay forcharacterizing multiple protein secretions from single cells. During the immunoassay, characteristic diffusion and kinetic time scalesis increased a hundred times from 0.313 to 31.3. Theoretical adsorption isotherms follow the trend of experimentally obtained data. Adsorption isotherms indicate that amount of proteins secreted from individual cells and subsequently captured on a clean glass slide surface increases monotonically with time. Our study directly validates that protein secretion rates can be quantified by the microengraving immunoassay. This will enable us to apply microengraving immunoassays to quantify secretion rates from 10⁴–10⁵ single cells in parallel, screen antigen-specific cells with the highest secretion rate for clonal expansion and quantitatively reveal cellular heterogeneity within a small cell sample.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.