A Cultivated Form of a Red Seaweed (Chondrus crispus), Suppresses β-Amyloid-Induced Paralysis in Caenorhabditis elegans

Marine Drugs

Jatinder Singh Sangha²  Owen Wally²  Arjun H. Banskota³  Roumiana Stefanova³  Jeff T. Hafting¹  Alan T. Critchley¹  Balakrishnan Prithiviraj² 

[1] Acadian Seaplants Limited, 30 Brown Avenue, Dartmouth, NS B3B 1X8, Canada; E-Mails:;Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, PO Box 550, Truro, NS B2N 5E3, Canada; E-Mails:;Aquatic and Crop Resources Development, National Research Council Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada; E-Mails:

关键词: β-amyloid;    Caenorhabditis elegans;    cultivated Chondrus crispus;    glycolipid;    monogalactosyl diacylglycerol (MGDG);    neuroprotection;    red seaweeds;   

DOI  :  10.3390/md13106407

来源: mdpi

【 摘 要 】

We report here the protective effects of a methanol extract from a cultivated strain of the red seaweed, Chondrus crispus, against β-amyloid-induced toxicity, in a transgenic Caenorhabditis elegans, expressing human Aβ_1-42 gene. The methanol extract of C. crispus (CCE), delayed β-amyloid-induced paralysis, whereas the water extract (CCW) was not effective. The CCE treatment did not affect the transcript abundance of amy1; however, Western blot analysis revealed a significant decrease of Aβ species, as compared to untreated worms. The transcript abundance of stress response genes; sod3, hsp16.2 and skn1 increased in CCE-treated worms. Bioassay guided fractionation of the CCE yielded a fraction enriched in monogalactosyl diacylglycerols (MGDG) that significantly delayed the onset of β-amyloid-induced paralysis. Taken together, these results suggested that the cultivated strain of C. crispus, whilst providing dietary nutritional value, may also have significant protective effects against β-amyloid-induced toxicity in C. elegans, partly through reduced β-amyloid species, up-regulation of stress induced genes and reduced accumulation of reactive oxygen species (ROS).

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.