High-Throughput Screening in Protein Engineering: Recent Advances and Future Perspectives

International Journal of Molecular Sciences

Magdalena Wójcik²  Aline Telzerow²  Wim J. Quax¹  Ykelien L. Boersma¹ 

[1] Groningen Research Institute of Pharmacy, Department of Pharmaceutical Biology, University of Groningen, A. Deusinglaan 1, 9717 AV Groningen, The Netherlands;

关键词: high-throughput screening;    FACS-based screening platforms;    microfluidics-based screening platforms;    protein engineering;    in vitro compartmentalization;   

DOI  :  10.3390/ijms161024918

来源: mdpi

【 摘 要 】

Over the last three decades, protein engineering has established itself as an important tool for the development of enzymes and (therapeutic) proteins with improved characteristics. New mutagenesis techniques and computational design tools have greatly aided in the advancement of protein engineering. Yet, one of the pivotal components to further advance protein engineering strategies is the high-throughput screening of variants. Compartmentalization is one of the key features allowing miniaturization and acceleration of screening. This review focuses on novel screening technologies applied in protein engineering, highlighting flow cytometry- and microfluidics-based platforms.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.